Effect of non-fiber carbohydrates on in vitro first order kinetics disappearance of cellulose

An in vitro experiment was conducted to determine the effect of supplemental non-fiber carbohydrate(NFC) on the disappearance kinetics of cellulose (Ce) by mixed ruminal microorganisms. Non-supplementedor NFC supplemented cellulose (467 mg NFC/g cellulose as sucrose (CeSu) or starch (CeSt) or a 1:1 mixtureof sucrose + starch (CeSuSt)) were incubated for 24, 48, and 96 h at 39°C. After each incubation time, pH,ammonia-N concentration and cellulose disappearance were measured. The disappearance kinetics rate of all samples was determined using first order exponential model of D(t) = D(i).exp (-k. time) + I; where D(t) is potentially digestible fraction; D(i) is potentially digestible residues; k is digestion rate of cellulose (h-1) and I is indigestible fraction. In this experiment, inclusion of NFC to the rumen fluid medium resulted in asignificant depression (P